Table 2.
Sequences of peptides used for the ELISA experiments and for the bifunctional nanoconstructs
| Peptides | Code | Amino acid sequencea |
|---|---|---|
| Fibrinβ60–74 | β60-74Arg | H-60RPAPPPISGGGYRAR74-NH2 |
| Fibrinβ60–74Cit60,72,74 | β60-74Cit | H-60 XPAPPPISGGGYXAX 74-NH2 |
| Biot-fibrinβ60–74 | Biot-β60-74Arg | Biot-60RPAPPPISGGGYRAR74−NH2 |
| Biot-fibrinβ60–74Cit60,72,74 | Biot-β60-74Cit | Biot-60 XPAPPPISGGGYXAX 74-NH2 |
| HIV-1 gp120233–247 Complement activating peptide | CNNK | Ac-233C(Acm)NNKTFNGTGPC(Acm)TNV247-NH2 |
| HIV-1 gp120233–247 Q-substituted complement activating peptide | CNNQ | Ac-233C(Acm)NNQTFNGTGPC(Acm)TNV247-NH2 |
| HIV-1 gp120233–247 Q-substituted complement activating peptide with C-terminal K | CNNQK | Ac-233C(Acm)NNQTFNGTGPC(Acm)TNV247-K-NH2 |
aStandard one-letter code for amino acid residues: X citrulline, Ac– acetyl group, –NH 2 amid group, Acm acetamidomethyl group, Biot biotinyl-aminohexanoyl group
ELISA enzyme-linked immunosorbent assay, HIV-1 human immunodeficiency virus type 1
Bold letters stand for modified amino acids that are not present in the natural sequences