Figure 4. 4E-BPs downregulation does not confer resistance to the MNK inhibitor CGP57380. (A) HEK cells were treated for 1 h with 10 or 20 µM of CGP57380 (CGP.) or cercosporamide (Cerco.) or left untreated. (B) HEK cells expressing shRNA against 4E-BP1 and/or 4E-BP2 or Scramble shRNA were treated with 20 μM of CGP57380 (CGP.) for 1 h or left untreated. (C) 4E-BP DKO and WT MEF were treated with 2.5 μM of PP242 or with 20 µM of CGP57380 for 1 h or left untreated. (D) MiaPaca-2 and Panc-1 cells expressing shRNA against the 3′UTR of MNK1 mRNA or Scramble shRNA were treated with 20µM of CGP for 1 h prior harvesting. Cell lysates were analyzed by western blotting using the indicated antibodies. Data are representative of at least three independent experiments.