Table 3. Comparison of experimental complexity, data analysis and cost.
| Protocol complexitya | Min. duration of experiment | Data analysis | Costb | |
|---|---|---|---|---|
| PUNCH-P | Medium | 2 d | t test/ANOVA | Low |
| pSILAC | Low | 2 d | t test/ANOVA | Low |
|
BONCAT/ QuaNCAT |
Medium | 2 d | t test/ANOVA | Low |
| Polysome-bound mRNA analysis | Medium | 3 d | Normalization, t test/ANOVA | Medium |
| Ribo-seq | High | 9 d | Complex | High |
| aLow complexity – requires basic cell culture and biochemical skills; Medium – requires additional ribosome isolation or click-chemistry techniques; High – requires expert biochemical and RNA handling skills and advanced knowledge in bioinformatics. | ||||