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. 2013 Apr 1;1(1):e24038. doi: 10.4161/trla.24038

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Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name ktrs-01-01-10924038-g008.jpg — Figure 8. PABP does not compete with eIF4A for binding to the eIF4G central region. In A and B, the amount of GST-eIF4G_601–1106, which contains the interaction domains for eIF4A and PABP, bound to glutathione Sepharose for each pull-down assay is shown in the Coomassie-stained gels (bottom panels). (A) Full-length eIF4A was added to each reaction in a 1:1 molar ratio to GST-eIF4G_601–1106 and His-PABP_1–393 was added in increasing amounts to the binding reactions. (B) His-PABP_1–393 was added to each reaction in a 1:1 molar ratio to GST-eIF4G_601–1106 and full-length eIF4A was added in increasing amounts to the binding reactions. The amount of (A) eIF4A or (B) PABP bound to GST-eIF4G_601–1106 was detected by Western analysis (top panels).