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. 2015 May 19;7(1):50–60. doi: 10.1111/1759-7714.12274

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© 2015 The Authors. Thoracic Cancer published by China Lung Oncology Group and Wiley Publishing Asia Pty Ltd.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Rad51 expression levels in H820 and A549 cells following IR. (a) Rad51 protein expression levels with (10 Gy) or without irradiation at different time points (0, 1, 12, and 24 hours following irradiation). (b) Levels of Rad51 protein in two cells at 12 hours after various doses of radiation. (c) Levels of Rad51 messenger ribonulcleic acid at 12 hours after radiation. Error bars, standard deviation (SD) from mean of triple independent measurements. Normalized fold expression: the relative expression of Rad51 divided by glyceraldehyde 3‐phopshate dehydrogenase; the expression level of Rad51 in A549 cell at 0 Gy was set as 1; *P < 0.05. (d) Levels of Rad51 protein in cytosolic and nuclear fractions with (10 Gy) or without irradiation at different time points (0 and 12 hours following irradiation). Blots were probed for β‐actin and lamin B to identify cytosolic and nuclear fractions, respectively. (c) () A549, () H820.

Inline graphic — Rad51 expression levels in H820 and A549 cells following IR. (a) Rad51 protein expression levels with (10 Gy) or without irradiation at different time points (0, 1, 12, and 24 hours following irradiation). (b) Levels of Rad51 protein in two cells at 12 hours after various doses of radiation. (c) Levels of Rad51 messenger ribonulcleic acid at 12 hours after radiation. Error bars, standard deviation (SD) from mean of triple independent measurements. Normalized fold expression: the relative expression of Rad51 divided by glyceraldehyde 3‐phopshate dehydrogenase; the expression level of Rad51 in A549 cell at 0 Gy was set as 1; *P < 0.05. (d) Levels of Rad51 protein in cytosolic and nuclear fractions with (10 Gy) or without irradiation at different time points (0 and 12 hours following irradiation). Blots were probed for β‐actin and lamin B to identify cytosolic and nuclear fractions, respectively. (c) () A549, () H820.