Figure 3.
Knockdown of Rad51 sensitizes non‐small cell lung cancer cells to IR. (a) Eighty‐four hours after transfection of the lentivirus, small hairpin ribonucleic acid vector successfully inhibited Rad51 expression with (10 Gy) or without irradiation (0 Gy) in H820 and A549 cells; (b) The survival fractions decreased after downregulation of Rad51; (c) Downregulation of Rad51 increased apoptotic cell fractions in both cell lines. Error bars, SD from mean of triple independent measurements. *P < 0.05; (d) Downregulation of Rad51 induced more residual γ‐H2AX
, indicating shrinkage of DNA repair function. Error bars, SD from mean of triple independent measurements. Normalized fold fluorescence intensity: the relative values of γ‐H2AX fluorescence intensity divided by unirradiated A549 cells. (e) Downregulation of Rad51 increased G2/M phase arrest and decreased S phase fractions. Column, mean values of triple independent experiments. (b) (
) A549‐nc, (
) H820‐nc, (
) A549 si‐rad51, (
) H820 si‐rad51; (c) (
) A549‐NC, (
) A549‐Rad51, (
) H820‐NC, (
) H820‐Rad51; (d) (
) A549‐NC, (
) H820‐NC, (
) A549‐Rad51, (
) H820‐Rad51; (e) (
) G2, (
) S, (
) G1. Si, small interfering; NC, negative control.