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. 2015 Dec 14;8(1):58–72. doi: 10.15252/emmm.201505496

Figure 2. Interaction of CHCHD10 with components of MICOS complex.

Figure 2

  1. Co‐immunoprecipitation (IP) of endogenous mitofilin, CHCHD10, and CHCHD3 in control and patient fibroblasts (1 mg of total extracts was used for each IP (left lower panel) and 200 μg was used for the input (left upper panel)). The same results were found in P2 fibroblasts (not shown). Reverse co‐IP experiment in HeLa cells with an antibody against CHCHD10 (right lower panel). 1 mg of total extracts was used for each IP and 200 μg was used for the input (right upper panel). The dividing lines correspond to gel sections visible in the raw data file.
  2. Duolink proximity ligation assay between mitofilin and CHCHD10 (upper panels), and CHCHD10 and CHCHD6 (lower panels) in control and patient fibroblasts observed by confocal microscopy. Mitochondria were stained with MitoTracker. Duolink spots per cell were quantified for 30 randomly selected individual cells per each studied fibroblast cell line from two independent experiments. Differences between the cell lines were analyzed by Student's t‐test (two‐sided): highly significant (***P = 0.0001). Scale bar = 10 μm.

Source data are available online for this figure.