Figure 8. Overexpression of the CHCHD10 mutant alleles in HeLa cells leads to inhibition of apoptotic cell death.

Transfections were performed with empty vector (EV) or vectors encoding either wild‐type CHCHD10‐FLAG (WT) or mutant CHCHD10‐FLAG (P34S and S59L).

• A
  Western blot on HeLa cell extracts using antibodies against FLAG or Hsp90.
• B–D
  HeLa cells transiently expressing the WT or mutated forms of CHCHD10‐FLAG (P34S or S59L) were treated with 1 μM actinomycin D (ActD) for 4, 6, or 8 h with measurement of annexin V/DAPI staining (B), DEVDase activity (C) from three independent experiments. Differences between the mutated and non‐mutated alleles were analyzed by Student's t‐test (two‐sided): significant (*: 0.05 > P > 0.01) or very significant (**: 0.01 > P > 0.001). P34S versus WT: **P = 0.0055 (4 h), *P = 0.0103 (8 h). S59L versus WT: *P = 0.0304 (4 h), *P = 0.0129 (8 h). Mitochondrial outer membrane permeabilization was determined by Western blot by assessing the degradation of SMAC (D). Hsp90 was used as a loading control.

Source data are available online for this figure.