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. 2016 Jan 15;95(2):e2491. doi: 10.1097/MD.0000000000002491

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Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.

This is an open access article distributed under the Creative Commons Attribution-ShareAlike License 4.0, which allows others to remix, tweak, and build upon the work, even for commercial purposes, as long as the author is credited and the new creations are licensed under the identical terms. http://creativecommons.org/licenses/by-sa/4.0

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Serial H&E (A) and BAP1 IHC (B) stained sections from an intrahepatic cholangiocarcinoma. The neoplastic cells demonstrate completely negative nuclear staining for BAP1 with preserved positive staining for endothelial cells (arrows) and lymphocytes (arrowheads) which serve as an internal positive control. In this case, there is nonspecific cytoplasmic staining in both the neoplastic and nonneoplastic cells. For the purposes of interpreting BAP1 IHC, this cytoplasmic staining is ignored (original magnification A, B ×400).