Tab. II.
Age-related positive samples for Giardia and Cryptosporidium by PCR and identification of genotypes as determined by sequencing of the TPI gene for Giardia and SSUrRNA gene for Cryptosporidium.
| Calves age | Giardia |
G. duodenalis genotypes (n) |
Cryptosporidium |
Cryptosporidium species (n) |
|---|---|---|---|---|
| No. of positive samples by PCR/No. of positive samples by IFA |
No. of positive samples by PCR/ No. of positive specimens by IFA |
|||
| 2-60 days | 25/34 | A (4); E (15)a | 10/10 |
C. ryanae (4) C. bovis (5) C. ubiquitum (1) |
| 61-150 days | 8/15 | A (1); E (6)b | 1/1 | C. bovis (1) |
| 151-240 days | 1/4 | E (1) | 1/1 | C. bovis (1) |
a
19/25 PCR products were successfully sequenced
b
7/8 PCR products were successfully sequenced