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. 2015 Nov 18;17(1):79–93. doi: 10.15252/embr.201540476

Figure EV1. Phenotypes associated with deletion of abo1 + .

Figure EV1

  1. Doubling time was estimated by measuring the OD595 of the indicated strains grown in YE5S medium at 30°C. Data are the mean of two independent biological repeats, and error bars denote the range of the data.
  2. abo1Δ cells have an elongated morphology. Microscopic analysis of DAPI‐stained cells. Data are representative of three biological repeats.
  3. The elongated morphology of abo1Δ cells is independent of the ATR checkpoint kinase, Rad3. Microscopic analysis of the indicated strain. Images are representative of duplicate experiments.
  4. An abo1Δ abo2Δ double mutant strain is not viable. Tetrad dissection of a genetic cross between abo1Δ and abo2Δ. Colonies arising from the spores of three asci are shown. Data are representative of three biological repeats.
  5. Subcellular localisation of Abo1. Cells expressing Abo1‐GFP were stained with DAPI and visualised using fluorescence microscopy. Data are representative of two biological repeats.