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. 2015 Nov 18;17(1):79–93. doi: 10.15252/embr.201540476

Figure EV3. Abo1 physically and genetically interacts with histones and FACT .

Figure EV3

  1. Deletion of the histone H3–H4 gene pair hht2 + –hhf2 + exacerbates phenotypes associated with deletion of abo1 +. The indicated strains were grown to mid log phase, subjected to five‐fold serial dilution and spotted onto YES5 plates supplemented as indicated. Plates were incubated for 2 days (37°C) or 3–5 days (30°C). Images are representative of three biological repeats. All strains were present on the same agar plates.
  2. Whole‐cell extracts prepared from the indicated strains were partially purified using IgG sepharose and analysed by Western blotting. Data are representative of two biological repeats.
  3. ChIP–qPCR analysis was used to detect Abo1‐GFP enrichment at the indicated loci in mid log‐phase cells grown at 30°C. Data are the mean of three independent repeats, and error bars denote ± SEM. P‐values were calculated using a two‐tailed unpaired t‐test. GFP‐tagged strains exhibit significant enrichment (P < 0.05) at both loci relative to the untagged control.
  4. Tetrad dissection of a genetic cross between abo1Δ and spt16‐18. The genotypes of colonies arising from the spores of five asci are shown. Analysis of a total of 28 tetrads from two independent genetic crosses failed to identify a viable double mutant strain.