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. 2015 Nov 23;17(1):64–78. doi: 10.15252/embr.201540809

Figure 8. Ubiquitinated AMOTL2 can bind to the LATS UBA domain.

Figure 8

  • A
    Schematics of human LATS1 and LATS2. PY, PY motif.
  • B, C
    293T cells were transfected with the indicated DNAs and immunoprecipitated with an anti‐Flag antibody or pulled down with streptavidin‐binding beads.
  • D, E
    293T cells were transfected as indicated and immunoprecipitated with an anti‐HA antibody. LATS2 immune complexes were subjected to a kinase assay with His‐YAP and cold ATP.
  • F
    Lats1 −/− ; Lats2 fl/fl ; SV40 mouse embryonic fibroblasts complemented with vector, LATS2 WT or ΔUBA mutant were infected with empty (control) or Zeocin‐Cre retroviruses and selected with 400 μg/ml Zeocin for 4 days. Harvested cells were analyzed by Western blotting for the indicated proteins.