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. 2015 Nov 12;17(1):27–36. doi: 10.15252/embr.201540614

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© 2015 The Authors. Published under the terms of the CC BY NC ND 4.0 license

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Figure EV3 — ApoE ACM‐treated human fibroblasts were analyzed using a Seahorse XF‐24 Flux Analyzer. Oxygen consumption rate (OCR) was determined (in pmol O₂/min/cell) after the addition of oligomycin (to inhibit ATP synthesis), FCCP (to uncouple ATP synthesis from respiration), and rotenone and antimycin A (to inhibit complexes I and III, respectively). The overall pattern of respiration was essentially similar between ApoE3 and ApoE4 ACM‐treated cells, although maximal respiration of ApoE4 ACM‐treated cells (the plateau between FCCP and rotenone/antimycin addition) was somewhat lower than that of ApoE3 ACM‐treated cells (mean ± SD, n = 3).