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Sample (100 μs) FRET efficiency histograms (FEHs) obtained with binning times of 50, 200, and 1000 μs and photon thresholds of 40, 120, and 450, respectively. FEHs were calculated using simulated data corresponding to 50 000 bursts assuming that the average residence time of the molecule on the confocal volume was 250 μs.
