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. 2016 Jan 19;11(1):e0147356. doi: 10.1371/journal.pone.0147356

Fig 1. ESAT-6/CFP-10 (E/C) peptides induced IL-21 production at the levels of mRNA and protein by PFCs in tuberculous pleurisy.

Fig 1

(A,B) PFCs were cultured in the presence of medium alone, with E/C peptides or with PMA plus ionomycin for 12 h, after which IL-21 and GAPDH mRNA levels were determined by RT-PCR (upper panel). The graph (lower panel) shows the ratio of IL-21 over GAPDH, calculated according to the relative intensities of the bands revealed under UV illumination with Bio-1D software. Data are representative of five separate experiments with similar results. (C) A representative data of ELISPOT results is shown. Data are representative of five separate experiments with similar results. (D) The frequency of IL-21-producing cells was enumerated by ELISPOT assay (n = 5). Each dot represents one donor. Horizontal bars represent the mean value. *p<0.05; **p<0.01; *** p<0.001.