Fig 2. E/C peptides induced IL-21 expression by CD4+ T cells from PFCs.
(A) Flow cytometric gating strategy used for analysis of lymphocytes from PFCs. (B) PFCs were stimulated in the presence of medium alone, with E/C peptides, or with PMA plus ionomycin. The expression of IL-21 was evaluated by FACS. Representative dot plots of thirty independent experiments are shown. (C) Statistical results of IL-21 expression by CD4+ and CD8+ T cells from PFCs are shown as the mean ± SEM. **p<0.01; *** p<0.001; N.S., not significant.