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. Author manuscript; available in PMC: 2016 Jan 19.
Published in final edited form as: J Cell Biochem. 2015 Jan;116(1):91–101. doi: 10.1002/jcb.24945

Fig. 2. EtOH increases gene expression and O-GLcNAc modification of Adamts1.

Fig. 2

C2C12 myocytes were incubated with EtOH as described in Fig.1. mRNA levels were determined by quantitative real-time PCR as described in the Materials and Methods section. Changes in mRNA level were calculated and normalized to rpL32 mRNA (Panel A). Panel B, equal amounts of cell lysates were immunoblotted with antibodies against Adamts1. Panel C, Cytosolic extracts (250–400 μg of protein) from control and EtOH-treated cells were incubated with WGA-conjugated beads overnight and analyzed for Adamts1 O-GLcNAc-modification. Results were normalized to total protein and expressed as a percentage of control levels. Data are means ± SE of 4 independent experiments consisting of 4 replicate samples per experiment. * P< 0.05 versus the control value.