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. Author manuscript; available in PMC: 2016 Jan 19.
Published in final edited form as: J Cell Biochem. 2015 Jan;116(1):91–101. doi: 10.1002/jcb.24945

Fig. 8. EtOH elevates sestrin 3 and its interaction with AMPK.

Fig. 8

C2C12 myocytes were incubated with EtOH as described in Fig.1. Equal amounts of protein from cell lysates were analyzed via Western blotting using antibody against sestrin3 (panel A). AMPK was immunoprecipitated from equal amount of cell extracts and immunoblotted with sestrin3 antibody. Results were normalized with immunoprecipitated (IP) AMPK that was assessed by immunoblotting (panel B). Data are means ± SE of 4 independent experiments consisting of 4 replicate samples per experiment. * P< 0.05 versus the control value.