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. 2016 Jan 19;11(1):e0146969. doi: 10.1371/journal.pone.0146969

Fig 2. Phosphorylation status of AMPK and GSK3β after media change with either 0.5 mM or 5.5 mM glucose.

Fig 2

After an overnight starve in serum-free, 0.5 mM glucose containing media, media was aspirated and fresh media was added containing either 0.5 or 5.5 mM glucose. Protein was harvested at the indicated time points. A) Addition of fresh, glucose-containing media significantly decreased AMPK phosphorylation (repeated measures ANOVA; time: p<0.01; time*treatment: 0.73; treatment: p = 0.082). B) Fresh media addition increased GSK3β phosphorylation (repeated measures ANOVA; time: p<0.001; time*treatment: p = 0.262; treatment: p = 0.424), although not significantly different between groups treated with either 0.5 or 5.5 mM glucose. Shown is densitometric analysis of the ratio of phosphorylated over total protein. Phosphorylation at 0 h was set to 100%. Note the different time points between pAMPK/AMPK and pGSK/GSK. Plotted are mean values ± SEM of 4 independent experiments.