Table 1.
mAb | Peptide | Ka (Ms−1) | Kd (s−1) | Kd (nM) |
---|---|---|---|---|
cis | pT231-tau | 40,700 | 1.10×10−5 | 0.27 |
cis | T231-tau | 0.17 | 1.00×10−5 | 58,820 |
trans | pT231-tau | 250 | 1.05×10−5 | 42.0 |
trans | T231-tau | 4.5 | 1.95×10−5 | 4,333 |
The association rate constant (Ka), dissociation rate constant (Kd), and binding constant (Kd) of cis and trans mAbs toward pT231- or T231-tau peptide were determined by Biacore analysis. Ms−1, millisecond; M, molar; s, second.