Fig 2. Phylogenetic relationships of the PA segment of AIVs in Peru.

Maximum likelihood phylogeny inferred for 2,184 PA segments of AIVs collected globally, including 27 that were collected from wild birds in Peru. For clarity, major AIV lineages have been simplified as cartoon triangles, although the branches for the Argentinian lineage are depicted as an inset. Major AIV clades are shaded accordingly to lineage, corresponding to Fig 1: Argentinian AIV lineage = purple; North American AIV lineages = red; global AIV lineage = green; Eurasian AIV lineage = blue. Bootstrap values > 70 are provided for key nodes, the tree is midpoint rooted for clarity, and all branch lengths are drawn to scale. Scale bar indicates number of nucleotide substitutions per site. The phylogenetic position of the 27 viruses from Peru is indicated with a solid black circle, along with the identifier and subtype for each virus. Viruses that cluster together are indicated by solid black circles that overlap, with bootstrap values provided in parentheses. Each cluster of overlapping black circles or individual black circle thus represents a putative viral introduction into Peru.