Table 1. Screening of HLA-A24:02-restricted epitopes from PEPP2.
| Peptide | Sequence | BIMAS score | SYPFPEITHI score | Binding MFI ratio | Stability MFI ratio |
|---|---|---|---|---|---|
| PEPP2194-202 | RALMARNML | 14.400 | 12 | 0.954 | 0.599 |
| PEPP2120-128 | RPQGAVGGL | 12.000 | 11 | 0.746 | 0.754 |
| PEPP2215-223 | AAEAITAPL | 8.400 | 11 | 0.803 | 0.665 |
| PEPP2188-196 | KWRRHQRAL | 8.000 | 11 | 0.772 | 0.714 |
| PEPP2141-149 | FTPLQLQEL | 7.920 | 12 | 0.860 | 0.687 |
| PEPP2271-279 | EFGPFPFVI | 6.000 | 19 | 1.153 | 1.201 |
| PEPP27-15 | CSQYMTSSL | 6.000 | 12 | 1.103 | 1.041 |
| PEPP2253-261 | SLPLPLMLL | 6.000 | 14 | 1.277 | 1.103 |
| PEPP227-35 | DMNAMVSL | 6.000 | 11 | 0.936 | 0.831 |
| PEPP2246-254 | MPPFPPPSL | 6.000 | 13 | 0.985 | 0.914 |
| PEPP281-89 | GGAGVPGHL | 5.600 | 13 | 0.852 | 0.785 |
| PEPP2117-125 | QYSRPQGAV | 5.000 | 10 | 0.882 | 0.977 |
| PEPP2231-239 | DYFWDHSHS | 5.000 | 12 | 0.889 | 0.869 |
| PEPP252-60 | QGTAAGEKL | 4.400 | 13 | 0.820 | 0.938 |
| PEPP2145-153 | QLQELERIF | 4.320 | 12 | 0.767 | 1.071 |
| PEPP26-14 | QCSQYMTSL | 4.000 | 10 | 0.724 | 0.614 |
| PEPP225-33 | LQDMNAMVL | 4.000 | 10 | 0.768 | 0.761 |
| PEPP2248-256 | PFPPPSLPL | 3.600 | 19 | 1.069 | 0.926 |
| PEPP2138-146 | VHAFTPLQL | 0.400 | 13 | 0.992 | 0.748 |
| PEPP2144-152 | LQLQELERI | 3.600 | 13 | 0.695 | 0.738 |
| PEPP2150-158 | ERIFQREQF | 0.300 | 13 | 0.747 | 0.690 |
| PEPP2277-285 | FVIVPSFTF | 3.000 | 13 | 1.181 | 0.928 |
| PEPP2140-149 | AFTPIQLQEL | 31.680 | 7 | 1.363 | 0.851 |
| PEPP29-18 | QYMTSLLSPA | 9.000 | 10 | 1.354 | 0.865 |
| EBV | TYGPVFMCL | 403.200 | 24 | 1.204 | 1.320 |
HLA-A*24:02-binding peptides derived from the PEPP2 protein were predicted using BIMAS and SYFPEITHI. Scores of those algorithms are indicated. HLA-A*24:02-binding peptide derived from EBV was used as a positive control. Mean-fluorescence intensity (MFI) ratio was calculated as shown in Materials and Methods. The average of the two assays is indicated.