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. 2016 Jan 19;11(1):e0147362. doi: 10.1371/journal.pone.0147362

Fig 7. Intracellular localization of SPARC protein by immunofluorescent staining and confocal microscopy.

Fig 7

SPARC (red) immunostaining as well as DAPI (blue) staining to identify all cells in the field is shown for the following UROtsa cell lines: (A) Parent; (B) Cd#4-DEST (blank vector); and (C) Cd#4-SPARC cells. Higher magnification images are shown for: (D) As#3-SPARC, (E) As#6-SPARC, and (F) Cd#1-SPARC cells. The inset shown in (E) for the As#6-SPARC cells shows a high magnification image of the region of cytoplasm indicated by the white box. This staining pattern is consistent with intracellular vesicles containing SPARC protein. Images in A-C correspond to bar in C (10μm), images in D-F correspond to the bar in F (10μm), and the bar shown in the inset corresponds to 2 μm.