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(A) Purity of Histone H1 and immunoprecipitated GST-tagged Ubl Parkin was evaluated by Coomassie staining. (B) In vitro phosphorylation assay using [γ-32P]-ATP, purified TcPINK1, and Ubl Parkin or Histone H1 shows that both are specifically phosphorylated by WT and not KI TcPINK1. WT TcPINK1 also displays autophosphorylation activity.
