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. 2016 Jan 19;11(1):e0146806. doi: 10.1371/journal.pone.0146806

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© 2016 Arterbery, Bogue

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Fig 1: Hhex^-/- ESCs show no defects when differentiated toward definitive endoderm. A) Analysis of FACS for SOX17 and GSC revealed that both Hhex^+/+ (blue) and Hhex^-/- (red) cultures produced similar percentages of differentiated DE cells. Light Scatter plot indicates the cells gated for sorting (green) and IGG plot (grey) confirms antigen specificity. B) Single channel and merged immunofluorescence staining of DE cultures after the 5-day culture. Both Hhex^+/+ and Hhex^-/- cultures showed significantly more cells double positive for the nuclear expression of SOX17 and FOXA2/HNF3β compared to IGG controls. (Scale bars = 100μM, *p<0.05, **p<0.01)