Fig 5. Effect of camphene on the incorporation of [¹⁴C]-acetate into de novo synthesized fatty acids.

On day 7, HepG2 cells were incubated for 24 h with camphene (25, 50 and 100 μM) in medium containing 10% (v/v) human LPDS. Four hours prior to the end of the incubation period, the cells were pulse-labeled with [¹⁴C]-acetic acid, sodium salt. The incorporation of acetate into fatty acids was determined as described. Results were normalized to the amount of cellular protein and expressed in percent of the respective control incubations. Results are from three independent experiments in triplicates and expressed as the mean ± SD. Significantly different compared to control: p<0.05 (*); p <0.001 (***) and ns (non significant) vs control by Student-Newmann-Keuls test.