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. 2016 Jan 19;11(1):e0147542. doi: 10.1371/journal.pone.0147542

Fig 5. Expression of CTEN rescues impaired cell adhesion caused by ΔNp63 deficiency.

Fig 5

RWPE-1 cells were transfected by control siRNA (si-ctrl), CTEN-specific siRNA (si-CTEN) or ΔNp63-specific siRNA (si-ΔNp63) together with FLAG-tagged CTEN expression plasmids (Flag-CTEN) or the corresponding empty vector. Cells were reseeded on plates coated with fibronectin 48 hr after transfection. Non-adherent cells were washed off by PBS and adherent cells were stained, solubilized and quantified by measuring the absorbance at 595 nm as described in materials and methods. The absorbance from each sample was normalized to that from the control (si-ctrl +; empty vector +) (upper panel). The final value is expressed as the mean relative fold change compared to the control. Data are expressed as the mean±standard deviation of three different experiments analyzed in triplicate. (*: P <0.005) Western analyses of ΔNp63 and CTEN expression were performed 48 hr after transfection. α-tubulin was used as a loading control. (lower panel)