Figure 7. Reduction in actomyosin activity suppresses the DC defects in embryos expressing the foscrb_Y10A variant.

(A) Quantification of the defects observed in cuticle preparations from the genotypes indicated in the graph. For the complete genotype see Figure 7—figure supplement 1. The category “DC defect” includes a range of defects ranging from cuticles of embryos that completed DC but do not hatch, to cuticles with large DC openings. The category “WT-like” includes all larvae that hatch. For details about the classifications see Figure 7—figure supplement 1. Note that all the genotypes have the foscrb_Y10A background, except the ones highlighted in magenta, numbers 18 and 19, that have the foscrb background. mean ± SD from 2–4 independent crosses. n = total number of cuticles counted for the indicated genotype. Note that suppression of the DC phenotype in foscrb_Y10A embryos is particularly evident upon expression of Flw-HA (10), Pak-AID (17), and DE-cad (22). (B-F) Adult flies of the indicated genotypes. In (F), the arrowhead marks the defects in the dorsal abdomen.

DOI: http://dx.doi.org/10.7554/eLife.07398.034

Figure 7—figure supplement 1. Reduction in the actomyosin activity suppresses the DC defects in embryos expressing the foscrb_Y10A variant.

Quantification of the defects observed in cuticle preparations from the genotypes indicated in the graph. In the category “Open cuticle”, the dorsal opening is so prominent that in some cases the mouthparts are exposed (arrowhead). Category “Dorsal hole” corresponds to those cuticles in which a medium (left picture) or small (right picture) dorsal hole is present, but the anterior part is closed. In the category “Closed but not hatched”, the closure is complete, the puckering of the epidermis is noticeable (arrowhead), but the larvae fail to hatch. In the category “Kinked larvae”, the puckering of the epidermis (arrowhead) results in larvae with the tail pointing upwards, so the larvae seem to have a kink. In the category “WT-like”, no defects are evident so the larvae are alike to wild type. mean ± SD from 2–4 independent crosses. n = total number of cuticles counted for the indicated genotype. For the statistical analysis see Table 1.

DOI: http://dx.doi.org/10.7554/eLife.07398.035

Figure 7—figure supplement 2. Phosphorylated DMoesin levels are reduced in embryos expressing the foscrb_Y10A variant.

Localisation of phospho-DMoesin (P-DMoe, A,B) in embryos at the beginning of stage 14. In all images the AS is at the top, for the genotypes w;foscrb;crb^GX24 and w;foscrb_Y10A;crb^GX24. The LE of foscrb_Y10Aembryo is marked with a magenta line (B). Scale bar: 10 μm. Representative images from 9 different embryos for each genotype.

DOI: http://dx.doi.org/10.7554/eLife.07398.036

Figure 7—figure supplement 3. Weak head phenotype of embryos expressing the foscrb_Y10A variant.

Examples of cuticles with a weak head phenotype: the arrows mark an opening in the anterior part.

DOI: http://dx.doi.org/10.7554/eLife.07398.037