Fig. 1.
Endothelial cells functionalized with polylactide-based MNP. Intracellular localization of MNP internalized by rat aortic endothelial cells over 24 h in presence of a high-gradient field (32.5 T/m) is visualized by fluorescent microscopy using particles stably labeled with BODIPY558/568 (merged bright field and red fluorescent image shown in A, original magnification ×400). Polylactide-based MNP formulated with 43% magnetite (w/w) exhibit strong magnetization at 1 kOe (uniform field strength used for in vivo studies) and a lack of remanence (B). Consistent with the particle magnetization pattern, MNP-functionalized cells show strong yet fully reversible magnetization (0.6 nemu per cell at 1 kOe) absent in control (unloaded) cells (C).