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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: J Control Release. 2015 Dec 17;222:169–175. doi: 10.1016/j.jconrel.2015.12.025

Fig. 3.

Fig. 3

In vitro disassembly analysis of MNP in the interior of contact-inhibited and dividing endothelial cells. MNP stably labeled with BODIPY558/568 exhibit complex excitation and emission patterns (A and B, respectively) suggesting partial aggregation of the fluorophore to form a species with red-shifted emission (λ = 612 nm) in intact MNP. Forced degradation of MNP with Proteinase K results in a readily quantifiable decrease in the ratio of fluorescence intensities at λ = 612 and 575 nm (C). This phenomenon was used for examining directly and non-invasively disassembly rates of polylactide-based MNP in the interior of contact-inhibited and dividing endothelial cells (D). Data in C and D are presented as mean ± SD.