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. 2016 Jan 7;5:e10147. doi: 10.7554/eLife.10147

Figure 1. Function and phylogeny of the guanylate kinase (gk) and GKPID protein family.

(A) The GKPID of the protein Discs-large (Dlg, blue) serves as a scaffold for spindle orientation by physically linking the localized cortical protein Pins (green) to astral microtubules (red) via the motor protein KHC-73 (black). (B) Reduced phylogeny of the protein family containing gk enzymes (brown) and protein-binding GKPIDs (blue). Parentheses show the number of sequences in each clade. Reconstructed proteins Anc-gkdup (the preduplication ancestor of gk enzymes and GKPIDs in animals/choanoflagellates), Anc-GK1PID and Anc-GK2PID (the GKPID in the common ancestor of animals and choanoflagellates, and of animals, respectively) are marked as circles with approximate likelihood ratio support. Scale bar indicates number of substitutions per site. For unreduced phylogeny, see Figure 1—figure supplement 1. Characteristics of the reconstructed sequences are found in Figure 1—figure supplement 2. For sequences analyzed, see Figure 1—source data 1. For sequences and posterior probabilities of amino acid states, see Figure 1—source data 2, Figure 1—source data 3.

DOI: http://dx.doi.org/10.7554/eLife.10147.003

Figure 1—source data 1. Species and identifiers for sequences used in alignment and phylogenetic analysis.
DOI: http://dx.doi.org/10.7554/eLife.10147.004
elife-10147-fig1-data1.xlsx (29.4KB, xlsx)
DOI: 10.7554/eLife.10147.004
Figure 1—source data 2. Posterior probability distribution of ancestral states for Ancgkdup.
DOI: http://dx.doi.org/10.7554/eLife.10147.005
elife-10147-fig1-data2.xlsx (52.5KB, xlsx)
DOI: 10.7554/eLife.10147.005
Figure 1—source data 3. Posterior probability distribution of ancestral states for AncGK1PID.
DOI: http://dx.doi.org/10.7554/eLife.10147.006
elife-10147-fig1-data3.xlsx (19.8KB, xlsx)
DOI: 10.7554/eLife.10147.006

Figure 1.

Figure 1—figure supplement 1. Complete phylogeny of 224 guanylate kinase enzyme and GKPIDs.

Figure 1—figure supplement 1.

Nodes are labeled with approximate likelihood ratio supports, and branch lengths are in substitutions per site (see scale bar). The tree is rooted on the bacterial GK enzymes. Major paralogs in the GKPID family, with the number of sequences included in each, are labeled. See Figure 1—source data 1 for species and accessions used. See Supplementary files 1 and 2 for alignment and tree file.
DOI: http://dx.doi.org/10.7554/eLife.10147.007

Figure 1—figure supplement 2. Sequence characteristics of maximum likelihood reconstructions of Anc-gkdup and Anc-GK1PID.

Figure 1—figure supplement 2.

(A,B) The histogram shows the distribution over sites of posterior probability support for maximum likelihood amino acid states (see Figure 1—source data 2, Figure 1—source data 3 for full sequences and support). (C) Sequence similarity of ancestral sequences to extant gk enzymes and GKPIDs. The table shows the percent of residues identical between each pair of sequences.
DOI: http://dx.doi.org/10.7554/eLife.10147.008