Table 1.
|
Regimen |
Marking system |
# Specimens |
# Marked DT |
HS stage |
HS |
DT/DB border clones |
DT/TC border clones |
|||
|
DT side |
DB side |
DT DB |
DT side |
TC side |
||||||
|
A |
GFP, NRE lacz |
106 |
40 |
48-50h AEL |
8' 35° |
3 |
0 |
5 |
0 |
|
|
B |
GFP, NRE-lacz |
59 |
24 |
24-32h AEL |
5' 37° |
7 |
1 |
8 |
0 |
|
|
C |
GFP |
29 |
11 |
24-32h AEL |
5' 37° |
2 |
1 |
1 |
1 |
|
|
D |
MARCM, α-Delta |
52 |
43 |
4-6h AEL |
60' 38° |
1 |
3 |
4 |
5 |
|
|
E |
MARCM, α-Cut |
48 |
34 |
4-6h AEL |
60' 38° |
6 |
2 |
6 |
5 |
|
|
F |
M MARCM, α-Ser |
251 |
30 |
4-6h AEL |
30' 38° |
3 |
2 |
2 |
3 |
|
|
G |
GFP & LacZ, α-Ser |
119 |
114 (GFP) 79 (LacZ)* |
24-26h AEL |
15' 37° |
11 |
11 |
6** |
8 |
0 |
|
H |
GFP & LacZ |
116 |
61 (GFP) 34 (LacZ)* |
24-26h AEL |
6' 37° |
9 |
5 |
0 |
6 |
1 |
|
TOTAL |
780 |
361 |
42 |
25 |
6 |
40 |
15 |
|||
Genotype A: NRElacz/hsFLP; actin>y >GAL4,UAS-GFP/ ; /MKRS B: NRElacz/hsFLP; actin>y >GAL4,UAS-GFP/ ; /MKRS C: hsFLP/Y; actin>y >GAL4,UAS-GFP/ ; /MKRS D: hsFLP122,tubGAL4,UAS-NLS-GFP/ ; tubGal80 FRT40a/FRT40a; / E: hsFLP122,tubGAL4,UAS-NLS-GFP/ ;tubGal80,FRT40a /FRT40a; / F: hsFLP,tubGAL4, UAS-GFP-NLS; / ;RpS17,tubGal80,FRT80a/FRT80a G: hsFLP/ orY; actin>y >GAL4,UAS-GFP/ ;actin>stop>lacZ-NLS/MKRS H: hsFLP/ orY; actin>y >GAL4,UAS-GFP/ ;actin>stop>lacZ-NLS/MKRS * # DTs with clones at or near the DB and TC borders ** specimens with independently marked DT and DB clones.