Table 2.

Method Validation Parameters for Measuring Carbonyls in Tobacco Smoke

		accuracy (n = 3)					relative standard deviation (n = 30)
analyte	calibration standard range (µg)	low	medium	high	smoke matrix effecta (n = 3)	LOD (µg) (n = 7)	3R4F ISOb	3R4F CIc
formaldehyde	5–300	106%	102%	99%	0%	2.4	19%	19%
acetaldehyde	50–3000	90%	100%	98%	−1.3%	2.7	10%	12%
acetone	25–1500	102%	99%	95%	3.1%	6.4	14%	14%
acrolein	5–300	100%	103%	95%	−7.5%	0.1	10%	13%
propionaldehyde	5–300	83%	102%	103%	−2.4%	0.6	13%	16%
crotonaldehyde	2.5–150	101%	101%	96%	−3.6%	0.2	12%	13%
methyl ethyl ketone	5–300	104%	93%	96%	−7.8%	0.3	14%	13%

^aMatrix effects were assessed by comparing slopes of calibration curves prepared from two set of standards. One set was prepared neat, and the second set was prepared with smoke matrices present. “+” or “−” indicates a matrix-enhanced or -suppressed effect, respectively.

^bISO (35 mL puff volume, 60 s puff interval, 2 s puff duration, and filter-tip vent open).

^cCI (55 mL puff volume, 30 s puff interval, 2 s puff duration, and 100% vent block).