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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Cell Biochem. 2015 Nov 20;412(0):73–79. doi: 10.1007/s11010-015-2609-6

Figure 3. VEGF-induced increases in endothelial barrier permeability require eNOS.

Figure 3

Panel A. L-NAME prevents VEGF-induced increases in endothelial barrier permeability. Cells in culture medium on filters were treated for 30 minute at 37°C with 60 μM L- or D-NAME as indicated, followed by addition of 100 ng/ml VEGF for another 30 minutes before assay of radiolabeled inulin permeability as described in Methods. Results are shown from 4 experiments, with bars not sharing the same small letters different at p < 0.05. Panel B. Sepiapterin inhibits VEGF-induced increases in endothelial permeability. HUVECs in culture medium on filters were treated at 37°C where indicated for 30 minutes with 30 μM sepiapterin, followed by 100 ng/ml VEGF. After 30 minutes the radiolabeled inulin transfer assay was carried out. Results are shown from 8 experiments, with not sharing the same small letters different at p < 0.05.