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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Hepatol. 2015 Oct 24;64(2):316–325. doi: 10.1016/j.jhep.2015.10.017

Fig. 6. Myca overexpression partially rescues the proliferation defect observed in JQ1-treated larvae.

Fig. 6

(A) Scheme illustrating the periods of Mtz and JQ1 treatment and the stages of heat-shock (red arrows). (B) Epifluorescence images showing fabp10a:CFP-NTR expression in the regenerating larvae at R30h (arrows) and their quantification. Numbers indicate the percentage of larvae showing the corresponding liver size (controls, n=63; hs:VP16-myca larvae, n=163). A graph shows the relative liver size, as assessed by hepatic CFP expression, between control and VP16-Myca-overexpressing larvae (controls, n=14; hs:VP16-myca larvae, n=20). (C) Scheme illustrating the periods of Mtz, JQ1, and EdU treatment and the stages of heat-shock (red arrow) and analysis (black arrow). (D) Single confocal optical section images of the regenerating liver at R12h processed for EdU labeling (green) and fluorescence detection of Tp1:H2B-mCherry (red). (E) Graph showing the percentage of EdU+ cells among H2B-mCherry+ cells as shown in (D). Error bars, ±SEM; scale bars, 100 μm.