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. Author manuscript; available in PMC: 2016 Jul 4.
Published in final edited form as: Nat Immunol. 2016 Jan 4;17(2):187–195. doi: 10.1038/ni.3327

Figure 2. Ins1eGFP mice ignore the eGFPp:I-Ab epitope.

Figure 2

(a) Contour plots of eGFPp:I-Ab-streptavidin-phycoerythrin (tet-PE) versus eGFPp:I-Ab-streptavidin-allophycocyanin (tet-APC) staining of tetramer-enriched CD4+ T cells from pooled spleens and lymph nodes of unimmunized WT or Ins1eGFP mice. Numbers indicate the percent of double tetramer-positive eGFPp:I-Ab-specific (double-tet+) cells.

(b) Numbers of double-tet+ CD4+ T cells from pooled spleens and lymph nodes of unimmunized WT and Ins1eGFP mice. Horizontal bars indicate geometric mean values and circles represent individual mice. Results are pooled from 5 experiments, n=16–17 total mice per group. (n.s. = not significant by unpaired t-test of log10 transformed data).

(c) Mean percent of double-tet+ CD4+ T cells from pooled spleens and lymph nodes of unimmunized WT or Ins1eGFP mice that were CD44lo. Data are pooled from 5 experiments, n=16–17 total mice per group. Error bars indicate standard deviations. (n.s. = not significant by unpaired t-test).