Figure 6. Expression of a self-epitope by thymic antigen-presenting cells induces intrathymic clonal deletion.

(a) Contour plots of tet-PE versus tet-APC staining of tetramer-enriched CD4⁺CD8⁻ thymocytes from WT or UBC^eGFP mice.

(b) Number of CD4⁺CD8⁻ double-tet⁺ cells in the thymuses of WT or UBC^eGFP mice. Horizontal bars indicate geometric mean values and circles represent individual mice. Data were pooled from two independent experiments, n=5 total mice per group. (* P < 0.0001 by unpaired t-test of log₁₀ transformed data).

(c) Contour plots of tet-PE versus tet-APC staining of tetramer-enriched CD4⁺ T cells from pooled spleens and lymph nodes of WT or UBC^eGFP mice.

(d) Number of CD4⁺ double-tet⁺ cells in pooled spleens and lymph nodes of WT or UBC^eGFP mice. Horizontal bars indicate geometric mean values and circles represent individual mice. Data were pooled from 4 independent experiments, n=10–17 total mice per group. Data for WT mice are replotted from Figure 2b. (* P < 0.0001 by unpaired t-test of log₁₀ transformed data).

Numbers on each plot in (a) and (c) indicate the percent of double-tet⁺ cells.