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. Author manuscript; available in PMC: 2017 Jan 19.
Published in final edited form as: Biochemistry. 2015 Dec 30;55(2):253–261. doi: 10.1021/acs.biochem.5b01171

Figure 4.

Figure 4

Effect of the variable linker region on RNA binding. PKRΔV binding to 40 bp dsRNA measured by sedimentation velocity. Data were obtained at 0.75 uM RNA and variable protein concentrations. (a) Normalized g(s*) distributions for samples containing 0 (black), 0.5 (blue), 1 (red), 3 (green) and 6 (tan) equivalents of PKRΔV. (b) Global analysis of difference curves for PKRΔV binding to 40 bp dsRNA using a model of three sequential binding events. The top panels show the data (points) and fit (lines) and the bottom panels show the residuals (points). The best fit parameters are presented in Table 1.