Figure 5. Methionine deficient diet in Gnmt-KO mice.
(A) Sudan red staining and quantification in liver from WT and Gnmt-KO mice fed with normal or methionine deficient diet (MDD). (B,C) Western blot from total protein from livers (B) and cytoplasmic proteins from hepatocytes (C) of WT and Gnmt-KO mice fed with MDD. (D) Percentage area of lipid bodies per hepatocyte in culture after O.A. treatment (1mM) for 8h. (E) BODIPY staining and percentage area of lipid bodies per cell in WT, Gnmt-KO hepatocytes, and Gnmt-KO hepatocytes cultured for 24h in methionine deficient medium (MDM). (Mean +/− SEM, t-test *p<0.05).