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Purification of NecC from T. discophora
| Purification step | Total protein (mg) |
Total activity (U) |
Specific activity (U/mg) |
Yield (%) |
Purification (fold) |
|---|---|---|---|---|---|
| Mycelium extract | 363.9 | 40.7 | 0.11 | 100 | 1.0 |
| Ammonium sulfate precipitation | 49.4 | 43.1 | 0.87 | 106 | 7.8 |
| DEAE chromatography | 14.9 | 10.0 | 0.67 | 25 | 6.0 |
