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Membrane fractions obtained by centrifugation of “crude” membranes in six-step sucrose gradient. The fractions were analysed for light scattering at λ = 600 nm (a), protein content measured with Bradford assay (b), protein content and NADH oxidase activity (c), and light scattering at λ = 450 nm and NPN fluorescence at λ = 405 nm (d). OM and IM symbols in panel (b) indicate outer membrane and inner membrane fractions respectively
