Fig. 3.

a Increased STAT1 phosphorylation in patients’ monocytes after IFN-α and IFN-γ treatment. PBMC from patients (solid line) and healthy controls (dashed lines) were stimulated for 15 min with IFN-α (500 U/ml) or IFN-γ (100 ng/ml). Cells were fixed and permeabilized prior to staining with anti-CD14 and anti-phospho-STAT1 (pY701) antibodies. The gate was set on CD14+ monocytes. b Increased STAT1 phosphorylation in patients’ CD4+ T cells following IFN-α and IL-27 stimulation. PBMC were stimulated with either IFN-α (10,000 U/ml) or IL-27 (200 ng/ml) for 7.5, 15 and 30 min. Cells were then treated as described in (a) and phospho-STAT1 levels were evaluated gating on CD4+ T cells (Red – unstimulated patient cells; blue – stimulated healthy control cells; orange – stimulated patient cells)