Fig. 1.

Sox6 suppression induced cell death in an RA dose-dependent manner. a P19[LacZ] and P19[Sox6] cells were cultured with various concentrations of RA for 48 h, then stained with trypan blue without fixation. Bars represent the mean ± SEM of three experiments in each group. b Cells were cultured with 500 nM RA for 48 h, then stained with Hoechst 33342 and PI for 30 min. Scale bar indicates 100 μm. Asterisk indicates differences that were considered to be significant at P < 0.05 by Scheffe’s F test