Fig. 3.

Sox6 suppression induced transcription, translation, and secretion of BMP-4, but not BMPRs. Cells were cultured in bacterial-grade dishes with or without 500 nM RA for 0–48 h, and then the mRNA expressions of a BMP-4 and d BMPRs were analyzed by RT-PCR. a To normalize for sample loading, the ratio of the value obtained in semi-quantitative determination of each BMP-4 band to the corresponding G3PDH band was used. BMP-4 protein levels were measured by ELISA using b cell lysates c and conditioned medium. Asterisk indicates differences that were considered to be significant at P < 0.05 by Scheffe’s F test