Table 5.
Phenotyping 45 Hanseniaspora sp. strains for enzymatic activities and growth ability.
| Substrate | Activity | Growth | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| β-glucosidase | Protease | Pectinase | Pectinase | Chitinase | Glycerol | 12°C | Anaerobia | CMI CuSO 4(μg/mL) | CMI Cu(OH)2 (μg/mL) | |
| Arbutin | Skim milk | PG Agar | PG Agarose | Chitin | – | – | – | – | – | |
| 516149 | LB | 1 | 0 | – | – | w | + | + | 4 | 4 |
| NZ1 | LB | 1 | 0 | – | – | – | NA | NA | NA | NA |
| NZ148 | LB | 0 | – | – | – | w | + | + | 4 | 2 |
| NZ15 | LB | 1 | – | – | – | w | + | + | 2 | 2 |
| NZ234 | LB | 0 | w | – | 1 | G | + | + | 2 | 4 |
| NZ5 | LB | 1 | 0 | – | – | – | + | + | 4 | 4 |
| TB Sau 1 | LB | 1 | – | – | – | w | + | + | 4 | 2 |
| TB Sem 1 | LB | 1 | 0 | – | – | w | + | + | 4 | 2 |
| IWBT Y1013 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1044 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1097 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1100 | LB | – | – | – | – | w | + | + | 2 | 4 |
| IWBT Y1116 | G | – | – | – | – | w | + | + | 2 | 4 |
| IWBT Y1133 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1139 | LB | – | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1173 | LB | – | – | – | – | w | + | + | 2 | 2 |
| IWBT Y1177 | LB | – | – | – | – | w | + | + | 2 | 2 |
| IWBT Y1190 | LB | – | – | – | – | w | + | + | 4 | 4 |
| IWBT Y1192 | LB | – | – | – | – | w | NA | NA | NA | NA |
| IWBT Y1196 | LB | – | – | – | – | w | NA | NA | NA | NA |
| Y-1612 | LB | 1 | – | – | – | w | + | + | 2 | 2 |
| Y-1614 | LB | 1 | 0 | – | – | w | – | – | 2 | 2 |
| IWBT Y861 | LB | 1 | – | – | 1 | w | + | + | 4 | 2 |
| IWBT Y864 | LB | 1 | – | – | – | w | + | + | 2 | 4 |
| IWBT Y888 | LB | – | – | – | – | w | + | + | 2 | 4 |
| Y-915 | LB | 1 | 0 | – | – | w | + | + | 4 | 2 |
| IWBT Y941 | LB | 0 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y952 | LB | 0 | – | – | – | w | + | + | 2 | 4 |
| IWBT Y966 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y967 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y968 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| IWBT Y969 | LB | – | – | – | – | w | NA | NA | NA | NA |
| YB-3199 | LB | 1 | 0 | – | – | w | + | + | 2 | 2 |
| YB-783 | LB | 4 | 0 | – | – | w | + | + | 2 | 4 |
| Yq NS2 | LB | 0 | 0 | – | – | w | + | + | 4 | 4 |
| Other Hanseniaspora sp. | ||||||||||
| H. guilliermondii 113816 | LB | 1 | – | – | – | w | + | + | 1 | 2 |
| H. guilliermondii IWBT Y901 | G | – | – | – | – | w | NA | NA | NA | NA |
| H. guilliermondii IWBT Y970 | G | – | – | – | – | w | NA | NA | NA | NA |
| H. guilliermondii IWBT Y1035 | LB | 1 | – | – | – | w | NA | NA | NA | NA |
| H. guilliermondii IWBT Y1165 | LB | – | – | – | – | w | NA | NA | NA | NA |
| H. guilliermondii Y-1625 | LB | 1 | 0 | – | – | w | – | + | 2 | 2 |
| H. opuntiae IWBT Y863 | LB | 0 | – | – | – | w | NA | NA | NA | NA |
| H. opuntiae IWBT Y875 | LB | – | – | – | – | w | NA | NA | NA | NA |
| H. vineae IWBT Y907 | LB | – | – | – | – | w | NA | NA | NA | NA |
| H. vineae IWBT Y971 | LB | – | – | – | – | w | NA | NA | NA | NA |
| CONTROLS | ||||||||||
| S. polymorphus CBS 8047 | 12 | 0 | G | – | 6 | G | NA | NA | NA | NA |
| S. paradoxus RO88 | G | 0 | 12 | W | – | G | NA | NA | NA | NA |
| M. pulcherrima IWBT Y1123 | 2 | 10 | G | – | W | G | + | + | 4 | 2 |
| M. pulcherrima IWBT Y1072 | 2 | 11 | G | – | W | G | NA | NA | NA | NA |
| S. cerevisiae VIN13 | G | – | W | – | – | G | NA | NA | NA | NA |
| M. chrysoperlae IWBT Y955 | 2 | W | G | – | 1 | G | NA | NA | NA | NA |
Zone sizes are indicated in mm, after subtracting the colony size from the total diameter of the zone; + or − indicates growth or absence of growth under these conditions; NA stands for “Not Available”; G indicates that there was colony growth but no extracellular enzyme activity was observed; W indicates very weak growth on the respective plate (single colonies could be observed within the spotted zone); LB indicates a color change in the colony, but no halo was observed.