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Increased tumor initiating potential of CAIX-enriched primary pancreatic tumor cells (PDX15). EpCAM+CAIX + and EpCAM+CAIX − sorted cells from PDX15 tumors were implanted at indicated numbers in RAG2 KO mice. (A) Growth curve of EpCAM+CAIX + and EpCAM+CAIX − phenotypes in RAG2 KO mice. Mice were implanted with 5000 cells of each population and were euthanized when tumor volume reached ~ 2000 mm3. (B) The gating strategy used to sort cells representing CAIX(+) or CAIX(−) phenotype.
