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. 2016 Jan 20;36(3):795–805. doi: 10.1523/JNEUROSCI.3413-15.2016

Figure 6.

Figure 6.

A, Representative double fluorescence immunohistochemical labeling of retrograde tracer (CTb deposited in RPa, green; Case 60) and audiogenic stress-induced Fos immunoreactivity (red) in rPH (3.3 mm posterior to bregma). Scale bar, 100 μm. Examples of single retrogradely labeled cells (green arrows), single Fos-labeled cells (red arrows), and cells expressing both markers (yellow arrows) are indicated, with the inset (A′; yellow box in A) providing magnified (40× objective) representation of single- and double-labeled processes. Scale bar, 20 μm. Counts + SEM of retrogradely labeled (Tracer), Fos-labeled (Fos), and double-labeled cells (Both) in rPH following tracer injections in the RPa (B) or PaMP (C). Approximately 34% of all retrogradely labeled cells from either the RPa or PaMP were also immunoreactive for Fos. n = 6 or 7 cases per condition.