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. 2016 Jan 20;36(3):872–889. doi: 10.1523/JNEUROSCI.3297-15.2016

Figure 2.

Figure 2.

HA size analysis by agarose gel electrophoresis and size exclusion chromatography. A, B, Agarose gel electrophoresis shows HA fragments (50–2500 kDa) in rabbit pup brain homogenates. HMW HA fragments (500–1000 kDa) are comparable between pups with and without IVH compared to controls without IVH at days 3, 7, and 14. Boxed area shows HMW HA fragments of 500–1000 kDa at day 3. C, D, Agarose gel electrophoresis was performed on brain homogenates from premature infant after digestion with hyaluronidase. HMW HA fragments (500–1000 kDa) are similar between infants with and without IVH in both white matter and GE. The bar charts shows mean ± SEM (n = 5 each group). E, F, Data are mean ± SEM (n = 3 pups each group). HA size was evaluated by size exclusion chromatography on samples from each of three groups of pups as indicated. Calibration was accomplished by running 2500, 1000, 500, 250, 100, and 10 kDa Select-HA standards. Comparisons between pups with and without IVH were insignificant at both days 3 and 7.