Table 1.

Oligonucleotides used in cloning of MD and CD variants of SLC4A11 and AE1

	Primer
Construct: Plasmid Name/Construct	Forward	Reverse
pSKL2 (HA-353-MD-SLC4A11)	5′-CTAGCTAGCGCCACCATGTACCCATACGATGTTCCGGATTACGCTAGGTTCCCCTTGTACCCCTTG-3′	5′-CCGCTCGAGTCAAGGCCTGTGCTCAGCGTC-3′
pSKL3 (HA-347-MD-SLC4A11)	5′-CTAGCTAGCGCCACCATGTACCCATACGATGTTCCGGATTACGCTCGGGAGGACATCGCACGCAGG-3′	5′-CCGCTCGAGTCAAGGCCTGTGCTCAGCGTC-3′
pSKL4 (HA-329-MD-SLC4A11)	5′-CTAGCTAGCGCCACCATGTACCCATACGATGTTCCGGATTACGCTAGACACCCAGAGCCCCCAAAG-3′	5′-CCGCTCGAGTCAAGGCCTGTGCTCAGCGTC-3′
pSKL5 (HA-307-MD-SLC4A11)	5′-CTAGCTAGCGCCACCATGTACCCATACGATGTTCCGGATTACGCTGTGAGCCACGGTCCAGTGGCG-3′	5′-CCGCTCGAGTCAAGGCCTGTGCTCAGCGTC-3′
pSKL6 (Myc-307-MD-SLC4A11)	5′-CTAGCTAGCGCCACCATGGAACAAAAGCTAATTTCAGAAGAAGACCTAGTGAGCCACGGTCCAGTGGCG-3′	5′-CCGCTCGAGTCAAGGCCTGTGCTCAGCGTC-3′
pSKL7 (HA-CD-SLC4A11)	5′-CTAGCTAGCGCCACCATGTACCCATACGATGTTCCGGATTACGCTAGCCAGGTCGGGGGGCGGGGAGAC-3′	5′-CCGCTCGAGTCATTTCCCAATAATGCCATCAGT-3′
pSKL8 (Myc-CD-SLC4A11)	5′-CTAGCTAGCGCCACCATGGAACAAAAGCTAATTTCAGAAGAAGACCTAAGCCAGGTCGGGGGGCGGGGAGAC-3′	5′-CCGCTCGAGTCATTTCCCAATAATGCCATCAGT-3′
pSKL9 (340-MD-AE1)	5′-CTAGCTAGCGCCACCAGGGAGCTACTTCGAAGGC-3′	5′-CCGCTCGAGTCACACAGGCATGGCCAC-3′
pSKL15 (HA-CD-300-SLC4A11)	5′-TGACTCGAGGACTGTGCCTTCTAGTTGCCAGC-3′	5′-ATGCACCAAGGCCTCCTTG-3′
pSKL16 (Myc-CD-300-SLC4A11)	5′-TGACTCGAGGACTGTGCCTTCTAGTTGCCAGC-3′	5′-ATGCACCAAGGCCTCCTTG-3′

Underlined bases code for hemagglutinin (HA) or Myc epitope tag. MD, membrane domain; CD, cytosolic domain.